The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar.
Identifieur interne : 000081 ( Main/Exploration ); précédent : 000080; suivant : 000082The Osmotin-Like Protein Gene PdOLP1 Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar.
Auteurs : Shaofeng Li [République populaire de Chine] ; Yaoxiang Zhang [République populaire de Chine] ; Xuebing Xin [République populaire de Chine] ; Changjun Ding [République populaire de Chine] ; Fuling Lv [République populaire de Chine] ; Wenjuan Mo [République populaire de Chine] ; Yongxiu Xia [République populaire de Chine] ; Shaoli Wang [République populaire de Chine] ; Jingyan Cai [République populaire de Chine] ; Lifang Sun [République populaire de Chine] ; Manyi Du [République populaire de Chine] ; Chenxi Dong [République populaire de Chine] ; Xu Gao [République populaire de Chine] ; Xinlu Dai [République populaire de Chine] ; Jianhui Zhang [États-Unis] ; Jinshuang Sun [République populaire de Chine]Source :
- International journal of molecular sciences [ 1422-0067 ] ; 2020.
Abstract
Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated PdOLP1 from Populus deltoides (Marsh.). Expression analysis showed that PdOLP1 transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in P. deltoides. We conducted phenotypic, anatomical, and molecular analyses of PdOLP1-overexpressing lines and the PdOLP1-downregulated hybrid poplar 84K (Populus alba × Populus glandulosa) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to PdOLP1, and are downregulated in PdOLP1-downregulated hybrid poplar 84K). The overexpression of PdOLP1 led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of PdOLP1-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in PdOLP1-downregulated lines. A PdOLP1 coexpression network was constructed and showed that PdOLP1 was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the PdOLP1 promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the PdOLP1 promoter in vivo. Together, our results suggest that PdOLP1 is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.
DOI: 10.3390/ijms21113993
PubMed: 32498411
PubMed Central: PMC7312728
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Jinshuang" uniqKey="Sun J" first="Jinshuang" last="Sun">Jinshuang Sun</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32498411</idno><idno type="pmid">32498411</idno><idno type="doi">10.3390/ijms21113993</idno><idno type="pmc">PMC7312728</idno><idno type="wicri:Area/Main/Corpus">000266</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000266</idno><idno type="wicri:Area/Main/Curation">000266</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000266</idno><idno type="wicri:Area/Main/Exploration">000266</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The Osmotin-Like Protein Gene <i>PdOLP1</i> Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar.</title><author><name sortKey="Li, Shaofeng" sort="Li, Shaofeng" uniqKey="Li S" first="Shaofeng" last="Li">Shaofeng Li</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Yaoxiang" sort="Zhang, Yaoxiang" uniqKey="Zhang Y" first="Yaoxiang" last="Zhang">Yaoxiang Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xin, Xuebing" sort="Xin, Xuebing" uniqKey="Xin X" first="Xuebing" last="Xin">Xuebing Xin</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ding, Changjun" sort="Ding, Changjun" uniqKey="Ding C" first="Changjun" last="Ding">Changjun Ding</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Lv, Fuling" sort="Lv, Fuling" uniqKey="Lv F" first="Fuling" last="Lv">Fuling Lv</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Mo, Wenjuan" sort="Mo, Wenjuan" uniqKey="Mo W" first="Wenjuan" last="Mo">Wenjuan Mo</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Xia, Yongxiu" sort="Xia, Yongxiu" uniqKey="Xia Y" first="Yongxiu" last="Xia">Yongxiu Xia</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Wang, Shaoli" sort="Wang, Shaoli" uniqKey="Wang S" first="Shaoli" last="Wang">Shaoli Wang</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Cai, Jingyan" sort="Cai, Jingyan" uniqKey="Cai J" first="Jingyan" last="Cai">Jingyan Cai</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Sun, Lifang" sort="Sun, Lifang" uniqKey="Sun L" first="Lifang" last="Sun">Lifang Sun</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Du, Manyi" sort="Du, Manyi" uniqKey="Du M" first="Manyi" last="Du">Manyi Du</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dong, Chenxi" sort="Dong, Chenxi" uniqKey="Dong C" first="Chenxi" last="Dong">Chenxi Dong</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Gao, Xu" sort="Gao, Xu" uniqKey="Gao X" first="Xu" last="Gao">Xu Gao</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dai, Xinlu" sort="Dai, Xinlu" uniqKey="Dai X" first="Xinlu" last="Dai">Xinlu Dai</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Zhang, Jianhui" sort="Zhang, Jianhui" uniqKey="Zhang J" first="Jianhui" last="Zhang">Jianhui Zhang</name><affiliation wicri:level="2"><nlm:affiliation>Department of Pharmaceutical Science, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University, Durham, NC 27707, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmaceutical Science, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University, Durham, NC 27707</wicri:regionArea><placeName><region type="state">Caroline du Nord</region></placeName></affiliation></author><author><name sortKey="Sun, Jinshuang" sort="Sun, Jinshuang" uniqKey="Sun J" first="Jinshuang" last="Sun">Jinshuang Sun</name><affiliation wicri:level="1"><nlm:affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">International journal of molecular sciences</title><idno type="eISSN">1422-0067</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated <i>PdOLP1</i> from <i>Populus deltoides</i> (Marsh.). Expression analysis showed that <i>PdOLP1</i> transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in <i>P</i>. <i>deltoides</i>. We conducted phenotypic, anatomical, and molecular analyses of <i>PdOLP1</i>-overexpressing lines and the <i>PdOLP1</i>-downregulated hybrid poplar 84K (<i>Populus alba</i> × <i>Populus glandulosa</i>) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to <i>PdOLP1</i>, and are downregulated in <i>PdOLP1</i>-downregulated hybrid poplar 84K). The overexpression of <i>PdOLP1</i> led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of <i>PdOLP1</i>-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in <i>PdOLP1</i>-downregulated lines. A <i>PdOLP1</i> coexpression network was constructed and showed that <i>PdOLP1</i> was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the <i>PdOLP1</i> promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the <i>PdOLP</i>1 promoter in vivo. Together, our results suggest that <i>PdOLP1</i> is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.</div></front></TEI><pubmed><MedlineCitation Status="In-Process" Owner="NLM"><PMID Version="1">32498411</PMID><DateRevised><Year>2020</Year><Month>06</Month><Day>27</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1422-0067</ISSN><JournalIssue CitedMedium="Internet"><Volume>21</Volume><Issue>11</Issue><PubDate><Year>2020</Year><Month>Jun</Month><Day>02</Day></PubDate></JournalIssue><Title>International journal of molecular sciences</Title><ISOAbbreviation>Int J Mol Sci</ISOAbbreviation></Journal><ArticleTitle>The Osmotin-Like Protein Gene <i>PdOLP1</i> Is Involved in Secondary Cell Wall Biosynthesis during Wood Formation in Poplar.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E3993</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/ijms21113993</ELocationID><Abstract><AbstractText>Osmotin-like proteins (OLPs) mediate defenses against abiotic and biotic stresses and fungal pathogens in plants. However, no OLPs have been functionally elucidated in poplar. Here, we report an osmotin-like protein designated <i>PdOLP1</i> from <i>Populus deltoides</i> (Marsh.). Expression analysis showed that <i>PdOLP1</i> transcripts were mainly present in immature xylem and immature phloem during vascular tissue development in <i>P</i>. <i>deltoides</i>. We conducted phenotypic, anatomical, and molecular analyses of <i>PdOLP1</i>-overexpressing lines and the <i>PdOLP1</i>-downregulated hybrid poplar 84K (<i>Populus alba</i> × <i>Populus glandulosa</i>) (Hybrid poplar 84K PagOLP1, PagOLP2, PagOLP3 and PagOLP4 are highly homologous to <i>PdOLP1</i>, and are downregulated in <i>PdOLP1</i>-downregulated hybrid poplar 84K). The overexpression of <i>PdOLP1</i> led to a reduction in the radial width and cell layer number in the xylem and phloem zones, in expression of genes involved in lignin biosynthesis, and in the fibers and vessels of xylem cell walls in the overexpressing lines. Additionally, the xylem vessels and fibers of <i>PdOLP1</i>-downregulated poplar exhibited increased secondary cell wall thickness. Elevated expression of secondary wall biosynthetic genes was accompanied by increases in lignin content, dry weight biomass, and carbon storage in <i>PdOLP1</i>-downregulated lines. A <i>PdOLP1</i> coexpression network was constructed and showed that <i>PdOLP1</i> was coexpressed with a large number of genes involved in secondary cell wall biosynthesis and wood development in poplar. Moreover, based on transcriptional activation assays, PtobZIP5 and PtobHLH7 activated the <i>PdOLP1</i> promoter, whereas PtoBLH8 and PtoWRKY40 repressed it. A yeast one-hybrid (Y1H) assay confirmed interaction of PtoBLH8, PtoMYB3, and PtoWRKY40 with the <i>PdOLP</i>1 promoter in vivo. Together, our results suggest that <i>PdOLP1</i> is a negative regulator of secondary wall biosynthesis and may be valuable for manipulating secondary cell wall deposition to improve carbon fixation efficiency in tree species.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Shaofeng</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Yaoxiang</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xin</LastName><ForeName>Xuebing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Changjun</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Key Laboratory of Tree Breeding and Cultivation, State Forestry Administration, Beijing 100091, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Fuling</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mo</LastName><ForeName>Wenjuan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Xia</LastName><ForeName>Yongxiu</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shaoli</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cai</LastName><ForeName>Jingyan</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Lifang</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Du</LastName><ForeName>Manyi</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Chenxi</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gao</LastName><ForeName>Xu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dai</LastName><ForeName>Xinlu</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Jianhui</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Pharmaceutical Science, Biomanufacturing Research Institute and Technology Enterprise, North Carolina Central University, Durham, NC 27707, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Jinshuang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Experimental Center of Forestry in North China, Chinese Academy of Forestry, Beijing 100023, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2020</Year><Month>06</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Int J Mol Sci</MedlineTA><NlmUniqueID>101092791</NlmUniqueID><ISSNLinking>1422-0067</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">PdOLP1</Keyword><Keyword MajorTopicYN="N">Populus deltoides</Keyword><Keyword MajorTopicYN="N">functional mechanisms</Keyword><Keyword MajorTopicYN="N">negative regulator</Keyword><Keyword MajorTopicYN="N">secondary wall biosynthesis</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year><Month>11</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2020</Year><Month>05</Month><Day>13</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2020</Year><Month>05</Month><Day>28</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>6</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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sort="Cai, Jingyan" uniqKey="Cai J" first="Jingyan" last="Cai">Jingyan Cai</name><name sortKey="Dai, Xinlu" sort="Dai, Xinlu" uniqKey="Dai X" first="Xinlu" last="Dai">Xinlu Dai</name><name sortKey="Ding, Changjun" sort="Ding, Changjun" uniqKey="Ding C" first="Changjun" last="Ding">Changjun Ding</name><name sortKey="Dong, Chenxi" sort="Dong, Chenxi" uniqKey="Dong C" first="Chenxi" last="Dong">Chenxi Dong</name><name sortKey="Du, Manyi" sort="Du, Manyi" uniqKey="Du M" first="Manyi" last="Du">Manyi Du</name><name sortKey="Gao, Xu" sort="Gao, Xu" uniqKey="Gao X" first="Xu" last="Gao">Xu Gao</name><name sortKey="Lv, Fuling" sort="Lv, Fuling" uniqKey="Lv F" first="Fuling" last="Lv">Fuling Lv</name><name sortKey="Mo, Wenjuan" sort="Mo, Wenjuan" uniqKey="Mo W" first="Wenjuan" last="Mo">Wenjuan Mo</name><name sortKey="Sun, Jinshuang" sort="Sun, Jinshuang" uniqKey="Sun J" first="Jinshuang" last="Sun">Jinshuang Sun</name><name sortKey="Sun, Lifang" sort="Sun, Lifang" uniqKey="Sun L" first="Lifang" last="Sun">Lifang Sun</name><name sortKey="Wang, Shaoli" sort="Wang, Shaoli" uniqKey="Wang S" first="Shaoli" last="Wang">Shaoli Wang</name><name sortKey="Xia, Yongxiu" sort="Xia, Yongxiu" uniqKey="Xia Y" first="Yongxiu" last="Xia">Yongxiu Xia</name><name sortKey="Xin, Xuebing" sort="Xin, Xuebing" uniqKey="Xin X" first="Xuebing" last="Xin">Xuebing Xin</name><name sortKey="Zhang, Yaoxiang" sort="Zhang, Yaoxiang" uniqKey="Zhang Y" first="Yaoxiang" last="Zhang">Yaoxiang Zhang</name></country><country name="États-Unis"><region name="Caroline du Nord"><name sortKey="Zhang, Jianhui" sort="Zhang, Jianhui" uniqKey="Zhang J" first="Jianhui" last="Zhang">Jianhui Zhang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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